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10X Genomics single cell transcriptomics
Single Cell Transcriptomics, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/single+cell+transcriptomes/bio_rxiv__64898__2026__06__01__729304-34-7-9
Average 86 stars, based on 1 article reviews
single cell transcriptomics - by Bioz Stars, 2026-09
86/100 stars

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Single Cell:

Article Title: TET1 facilitates specification of early human lineages including germ cells
Article Snippet: .. To determine which of the three TET enzymes are expressed by hPGCLCs, we used the induction of hPGCLCs from hESCs by differentiating MEF-cultured hESCs into incipient mesoderm-like cells (iMeLCs), followed by differentiation of the iMeLCs as three dimensional (3D) aggregates in low adhesion 96-well plates in the presence of BMP4 and other cytokines to generate hPGCLCs ( A)., , Using previously published single cell transcriptomes generated with 10X Genomics technology, we show that TET1 mRNA is the only member of the TET family expressed at detectable levels in aggregate cells including the hPGCLCs (shown is UCLA1 in B and 1C and UCLA2 in A and S1B). ..

Article Title: The cellular basis of distinct thirst modalities
Article Snippet: .. Cell suspension volumes estimated to retrieve ~10 000 single cell transcriptomes were added to the 10x Genomics RT reaction mix and loaded to the 10X Single Cell B chip (10x Genomics #1000074) per manufacturer’s protocol. .. We used the ChromiumTM Single Cell 3’ GEM, Library & Gel Bead Kit v3.0 (10x Genomics #1000075) and Chromium i7 Multiplex Kit (#120262) to prepare Illumina sequencing libraries downstream of reverse transcription following manufacturer’s protocol applying 11 rounds of cDNA library amplification and 12 rounds of sequencing library amplification applied to 70 ng of cDNA library input to library synthesis.

Generated:

Article Title: TET1 facilitates specification of early human lineages including germ cells
Article Snippet: .. To determine which of the three TET enzymes are expressed by hPGCLCs, we used the induction of hPGCLCs from hESCs by differentiating MEF-cultured hESCs into incipient mesoderm-like cells (iMeLCs), followed by differentiation of the iMeLCs as three dimensional (3D) aggregates in low adhesion 96-well plates in the presence of BMP4 and other cytokines to generate hPGCLCs ( A)., , Using previously published single cell transcriptomes generated with 10X Genomics technology, we show that TET1 mRNA is the only member of the TET family expressed at detectable levels in aggregate cells including the hPGCLCs (shown is UCLA1 in B and 1C and UCLA2 in A and S1B). ..

Article Title: Functional BRI2-TREM2 interactions in microglia: implications for Alzheimer’s and related dementias
Article Snippet: .. Single/live CD11b + cells were isolated from WT control, Itm2b-KO , Trem2-KO and Itm2b/Trem2-dKO (double KO) brains, and single-cell transcriptomes were generated using the 10x Genomics platform in two independent experiments. ..

Article Title: Loss of ATG7 in microglia impairs UPR, triggers ferroptosis, and weakens amyloid pathology control.
Article Snippet: Microglia impact brain development, homeostasis, and pathology.. One important microglial function in Alzheimer’s disease (AD) is to contain proteotoxic amyloid-β (Aβ) plaques.. Recent studies reported the involvement of autophagy-related (ATG) proteins in this process.

Article Title: BRI2-mediated regulation of TREM2 processing in microglia and its potential implications for Alzheimer’s disease and related dementias
Article Snippet: .. Single/live CD11b + cells were isolated from WT control, Itm2b-KO, Trem2-KO and Itm2b/Trem2-dKO (double KO) brains, and single-cell transcriptomes were generated using the 10x Genomics platform in two independent experiments. ..

Isolation:

Article Title: Functional BRI2-TREM2 interactions in microglia: implications for Alzheimer’s and related dementias
Article Snippet: .. Single/live CD11b + cells were isolated from WT control, Itm2b-KO , Trem2-KO and Itm2b/Trem2-dKO (double KO) brains, and single-cell transcriptomes were generated using the 10x Genomics platform in two independent experiments. ..

Article Title: BRI2-mediated regulation of TREM2 processing in microglia and its potential implications for Alzheimer’s disease and related dementias
Article Snippet: .. Single/live CD11b + cells were isolated from WT control, Itm2b-KO, Trem2-KO and Itm2b/Trem2-dKO (double KO) brains, and single-cell transcriptomes were generated using the 10x Genomics platform in two independent experiments. ..

Control:

Article Title: Functional BRI2-TREM2 interactions in microglia: implications for Alzheimer’s and related dementias
Article Snippet: .. Single/live CD11b + cells were isolated from WT control, Itm2b-KO , Trem2-KO and Itm2b/Trem2-dKO (double KO) brains, and single-cell transcriptomes were generated using the 10x Genomics platform in two independent experiments. ..

Article Title: BRI2-mediated regulation of TREM2 processing in microglia and its potential implications for Alzheimer’s disease and related dementias
Article Snippet: .. Single/live CD11b + cells were isolated from WT control, Itm2b-KO, Trem2-KO and Itm2b/Trem2-dKO (double KO) brains, and single-cell transcriptomes were generated using the 10x Genomics platform in two independent experiments. ..

Suspension:

Article Title: The cellular basis of distinct thirst modalities
Article Snippet: .. Cell suspension volumes estimated to retrieve ~10 000 single cell transcriptomes were added to the 10x Genomics RT reaction mix and loaded to the 10X Single Cell B chip (10x Genomics #1000074) per manufacturer’s protocol. .. We used the ChromiumTM Single Cell 3’ GEM, Library & Gel Bead Kit v3.0 (10x Genomics #1000075) and Chromium i7 Multiplex Kit (#120262) to prepare Illumina sequencing libraries downstream of reverse transcription following manufacturer’s protocol applying 11 rounds of cDNA library amplification and 12 rounds of sequencing library amplification applied to 70 ng of cDNA library input to library synthesis.



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